Centrifuge Safety Manual-General Safety Measures Part 3
General Safety Measures
3. Sample management is also very important to safety. Lack of care can result in
exposure of the user to harmful materials.
Always use sample tubes or bottles designed for the particular rotor being used.
In general, samples should be capped to avoid generation of aerosols.
Nitrocellulose tubes should only be used when transparent and flexible.
They must never be heated because of explosion possibility.
Plastic centrifuge tubes should be discarded after one cycle of ultracentrifugation. The
failure rate for used tubes is a hazard that justifies using new tubes for each high G run.
When using radioactive, toxic, or pathogenic materials, be aware of potential hazards
associated with them in case of leakage during centrifugation. If leakage does occur
you may be exposed to particles dispersed in the air (aerosol). It is recommended that
additional precautions be taken to prevent exposure to these materials such as the use
of controlled ventilation or isolation areas.
If exposure occurs to radioactive, toxic or pathogenic materials all necessary
precautions and appropriate decontamination procedures should be used
(see lab safety procedures for toxic/pathogenic and Radiation Safety Guide for
radioactive).
Dispose of all waste solutions according to appropriate health and safety guidelines.
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