Centrifuge tubes and Microcentrifuge tube
Published:10-03-11 09:39 write:kd Click:
Centrifuge tubes
Centrifuge tubes or centrifuge tips are tapered tubes of various sizes made of glass or plastic. They may vary in capacity from tens of millilitres, to much smaller capacities used in microcentrifuges used extensively in molecular biology laboratories. The most commonly encountered tubes are of about the size and shape of a normal test tube (~ 10 cm long). Microcentrifuges typically accommodate microcentrifuge tubes with capacities from 250 μl to 2.0 ml. These are exclusively made of plastic.
Glass centrifuge tubes can be used with most solvents, but tend to be more expensive. They can be cleaned like other laboratory glassware, and can be sterilized by autoclaving. Plastic centrifuge tubes, especially microcentrifuge tubes tend to be less expensive. Water is preferred when plastic centrifuge tubes are used. They are more difficult to clean thoroughly, and are usually inexpensive enough to be considered disposable.
Microcentrifuge tube
Microcentrifuge tube with Coomassie Blue solution.
Microcentrifuge tubes or microfuge tubes are small, cylindrical plastic containers with conical bottoms, typically with an integral snap cap. They are used in molecular biology and biochemistry to store and centrifuge small amounts of liquid. As they are inexpensive and considered disposable, they are used by many chemists and biologists as convenient sample vials in lieu of glass vials; this is particularly useful when there is only a small amount of liquid in the tube or when small amounts of other liquids are being added, because microcentrifugation can be used to collect the drops together at the bottom of the tube after pipetting or mixing.
Made of polypropylene, they can be used in very low temperature (-80 °C to liquid nitrogen temperatures) or with organic solvents such as chloroform. They come in many different sizes, generally ranging from 250 μL to 2.0 mL. The most common size is 1.5 mL. Disinfection is possible (1 atm, 120 °C, 20 minutes) and is commoly performed in works related to DNA or microbes, where purity of the sample is of utmost importance. Due to their low cost and the difficulty in cleaning the plastic surface, they are usually discarded after each use.
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